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Dr. Workenesh Ayele Moltotal

Dr. Workenesh Ayele Moltotal

Researcher I, Virology and Rickettsiology Research Team
  • Academic qualification:
  • BSc, MSc, PhD
  • Research focus:
  • Previous experience working in the area of HIV/AIDS, hepatitis C, rabies, measles and more currently, on influenza and other respiratory viruses, and viral diseases of zoonotic origin with significant public health impact
  • Area of expertise :
  • evaluation and validation of diagnostic techniques for diseases of public health importance, application of established diagnostic technologies to support disease surveillance programs and/or design and execution of research projects
  • Address:
  • Tel.: +251-11-2788059
  • Email: workenesha@ehnri.gov.et, wayele@gmail.com
  • P.O.Box 1242, Addis Ababa, Ethiopia
Publications

  • Ayele W, Mekonnen Y, Messele T, Mengistu Y, Tsegaye A, Bakker M, Berkhout B, Dorigo-Zetsma W, Wolday D, Goudsmit J, Coutinho R, de Baar M, Paxton WA, Pollakis G. Differences in HIV Type 1 RNA Plasma Load Profile of Closely Related Co-circulating Ethiopian Subtype C Strains: C and C’.  AIDS Res Hum Retroviruses 2010; 26(7):805-13. 
  • Ayele W, Assefa T, Lulseged S, Tegbaru B, Berhanu H, Tamene W, Ahmedin Z,  Tensai  BW, Tafesse M, Mengistu Y, Goudsmit J, De Baar MP, Messele T, Pollakis G. RNA Detection and Subtype C assessment of HIV-1 in infants with Diarrhea in  Ethiopia. Open AIDS J 2009; 3:19-23.
  •  Ayele W, Schuurman R, Messele T, Dorigo-Zetsma W, Mengistu Y, Goudsmit J, Paxton WA, de Baar MP, Pollakis G. Use of dried spots of whole blood, plasma, and mother’s milk collected on       filter paper for measurement of human immunodeficiency virus type 1 burden. J Clin  Microbiol         2007; 45(3):891-6.
  • Ayele W, De Baar MP, Goudsmit J, Kliphuis A, Tilahun T, Dorigo-Zetsma W, Wolday D, Abebe A, Mengistu Y, Pollakis G. Surveillance technology for HIV-1 subtype C in Ethiopia: an env-based NASBA-molecular beacon assay to discriminate between subcluster C and C’.  J Virol Methods 2005; 130(1-2):22-9.
  • Adal M, Ayele W, Wolday D, Dagne K, Messele T, Tilahun T, Berkhout B, Mayaan S, Pollakis G, Dorigo-Zetsma W. Evidence of genetic variability of human immunodeficiency virus type 1 in Plasma and cervicovaginal lavage in Ethiopian women seeking care for sexually transmitted infections. AIDS Res Hum Retroviruses 2005; 21(7):649-53.
  • Ayele W, Pollakis G, Abebe A, Fisseha B, Tegbaru B, Tesfaye G, Mengistu Y, Wolday D, van Gemen B, Goudsmit J, Dorigo-Zetsma W, De Baar MP. Development of a nucleic acid sequence-based amplification assay that uses gag-based molecular beacons to distinguish between human immunodeficiency virus type 1 subtype C and C’ infections in Ethiopia. J Clin Microbiol 2004; 42(4):1534-41.
  • Enquselassie F, Ayele W, Dejene A, Messele T, Abebe A, Cutts FT, Nokes DJ. Seroepidemiology of measles in Addis Ababa, Ethiopia: implications for control through vaccination. Epidemiol Infect 2003;130(3):507-19.
  • Ayele W, Nokes DJ, Abebe A, Messele T, Dejene A, Enquselassie F, Rinke de Wit TF, Fontanet AL.  Higher prevalence of anti-HCV antibodies among HIV-positive compared to HIV-negative inhabitants of Addis Ababa, Ethiopia. J Med Virol 2002;68(1):12-7.
  • Ayele W, Fekadu M, Zewdie B, Beyene M, Bogale Y, Mocha K, Egziabher FG.Immunogenicity and efficacy of Fermi-type nerve tissue rabies vaccine in mice and in humans   undergoing post-exposure prophylaxis for rabies in Ethiopia. Ethiop Med J 2001;39(4):313-21.
  • Benvenuto JA, Ayele W, Legha SS, Raber MN, Nicaise C, Newman RA. Clinical pharmacokinetics of ifosfamide in combination with N-acetylcysteine. Anticancer Drugs 1992;3(1):19-23.
  • Newman RA, Siddik ZH, Travis EL, Followil D, Ayele W, Burditt T, Krakoff IH. Assessment of pulmonary and hematologic toxicities of liblomycin, a novel bleomycin analog. Invest New Drugs 1990;8(1):33-41.
  • Horan KL, Adamski SW, Ayele W, Langone JJ, Grega GJ. Evidence that prolonged histamine suffusions produce transient increases in vascular permeability subsequent to the formation of venular macromolecular leakage sites:Proof of the Majno-Palade hypothesis. Am J Pathol 1986; 123(3):570-6.
Abstracts/Presentations  
  •  Ayele W, Demissie G, Kassa W et al. “A Review of Influenza Sentinel Surveillance in Ethiopia: Virological Findings Year I”. Accepted for a poster presentation at the 2010 CDC International Influenza Grantee and Burden of Disease Meeting, 9-11 July 2010, Atlanta, Georgia (USA).
  • Ayele W, Demissie G, Seifu S et al. “Primer and Probe Sequence Homologies in real-time PCR-based Diagnosis of Different Influenza A Subtypes: Implications for Proficiency Panel Testing”. Oral presentation at the Biological Society of Ethiopia, 20th Annual Conference, Addis Ababa, Ethiopia (5-6 March 2010).      
  • Ayele W, Seifu S, Demissie G et al. “Low Probability, High Impact Public Health Events: The Case of New Influenza A(H1N1) in Ethiopia”. Oral presentation at the 2009 Africa Influenza Scientific Symposium, National Institute of Communicable Diseases, Johannesburg, South Africa (7-9 December, 2009).
  • Deressa A, Ayele W, Gisso A, Lemi T, Wendabeku M, Sang R. Assessing the threat of Rift Valley Fever (RVF) epidemics at the Ethio-Kenya border. Oral presentation at the 43rd Annual Medical Conference of the Ethiopian Medical Association, Addis Ababa, Ethiopia (June 2007).
  • Ayele W, Asfaw B, Abebe A et al. Monitoring the evolution of viral strains: implications for the control of pandemic influenza. Oral presentation at the 17th annual conference, Biological Society of Ethiopia, Addis Ababa (February 2007) .
  • Ayele W, Abebe A, Asfaw B et al. Establishing influenza surveillance in Ethiopia: making  the  case in a scenario of competing priorities. Oral presentation at the 17th annual conference, Ethiopian Public Health Association, Harer, Ethiopia (October 2006).
  • Ayele W. Avian Influenza A/H5N1: Next Pandemic Virus? Properties & Diagnosis. Plenary session paper presentation at 20th annual conference, Ethiopian Veterinary Association, Addis Ababa, Ethiopia (April 2006).
  • Ayele W, Yimer E, Abebe A, Mohammed E, Ismail S, Mengistu Y, Tareke I, Tesfahun A, Wuhib T, Messele T. Lab-based diagnosis of Influenza H5N1 in Ethiopia:Prospects  and Challenges. Oral  presentation at 42nd annual conference, Ethiopian Medical Association, Addis Ababa (June 2006).
  • Ayele W, Wolday D, Mekonnen Y et al. Variance of co-circulating subtype C strains reflecting  differences in incidence and disease progression. Poster presentation at 3rd International AIDS Society (IAS) Conference on HIV Pathogenesis and Treatment, Rio de Janeiro, Brazil (July 2005).
  • Ayele W,  Pollakis G, Goudsmit J, Kliphuis A, Tilahun T,  Dorigo-Zetsma W, Wolday D, Abebe A, Mengistu Y, de Baar MP. A Subtyping Approach for HIV-1 in Ethiopia: An Env-based  Assay to Distinguish Between  HIV-1 Subtype C / subcluster  C and C'. Oral presentations at 25th African Health Sciences Congress, Nairobi, Kenya (4-8th 0ctober 2004) & at 15th annual conference of  the Ethiopian Public  Health Association, Gondar, Ethiopia (15-17th  October 2004).
  • Ayele W, Assefa T, Tegbaru B, Messele T, Berhanu H, Tamene W, Ahmedin Z, Lulseged S,W/Tensai B, Taffesse M, Mengistu Y, de Baar MP, Pollakis G, Goudsmit J. Established HIV infection  in HIV-seropositive children 15 months or below presenting with diarrhea in 3 Addis Ababa hospitals and assessment of HIV-1 subtype C genotype in infected children. Oral presentation at 40th annual conference, Ethiopian Medical Association, Addis Ababa (June 2004).
  • Ayele W,  Dorigo-Zetsma W, Pollakis G, Wolday D, Meles H, Girma M, Messele T, Sanders E, van Gemen B, Mengistu Y, Goudsmit J, de Baar MP. Preliminary investigation of using dried blood spots as a method of evaluating viral load in HIV-infected Ethiopians. Oral presentation at 39th annual conference, Ethiopian Medical Association, Addis Ababa (May 2003).
  • Ayele W, de Baar MP, Abebe A, Mengistu Y, Dorigo-Zetsma W, Wolday D, van Gemen B,Pollakis G, Goudsmit J. Development of a GAG-based NASBA molecular beacon assay to distinguish between subtype C and C' HIV-1 in Ethiopia: potential for intervention strategies. Oral presentation at 38th annual conference, Ethiopian Medical Association, Addis Ababa (May 2002) and poster presentation at 14th International AIDS Conference, Barcelona, Spain (July 2002).
  • Ayele W, Abebe A, Messele T, Fontanet A, Dejene A, Enquselassie F, Nokes DJ.  Five times higher prevalence of anti-HCV antibodies among HIV-1 positive compared to HIV-1 negative inhabitants of Addis Ababa: Results from a community based survey. Oral presentations at the 13th International AIDS Conference, Durban South Africa (July 2000), 1st International Conference on AIDS in Ethiopia, Addis Ababa (November 1999) and 10th annual conference of the Ethiopian Public Health  Association, Addis Ababa (October 1999).
  • Ayele W, Fekadu M,  Zewdie B, Beyene M, Bogale Y, Fissehatsion G/Egziabher, Mocha K. Determination of the immunogenicity of Fermi type antirabies vaccine in an animal model. Oral presentation, 9th annual conference, Biological Society of Ethiopia (February 1999).
  •  Engineer MS, Ayele W, Legha SS, Newman RA. Clinical Pharmacology of Ifosfamide.Presentation at American Association for Cancer Research, 80th annual meeting (1989).

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